• Learn how O-PTIR has been uniquely utilized to track DNL, in space (with submicron resolution) and time, as isotopically labelled glucose is converted to lipids in live metabolically active cells in water (non-deuterated).

Additionally, Dr Mustafa Kansiz, Director of Product Management at Photothermal Spectroscopy Corp, will provide a brief technique overview, covering submicron simultaneous IR+Raman with co-located fluorescence widefield imaging, with advances now delivering sub-500nm IR spatial resolution. Learn how;

• O-PTIR achieves the same high spatial resolution of Raman microscopy, but with the rich information content of IR spectroscopy (eg protein secondary structure) with high sensitivity in seconds
• O-PTIR eliminates many traditional challenges of Raman microscopy, such poor sensitivity of cells and fluorescence interferences.
• O-PTIR enables exploration of the synergistic complementarity between IR and Raman while allowing the user to collect submicron IR and Raman spectra simultaneously, from the same spot, at the same time with the same spatial resolution. These breakthrough advances will be presented together with a range of biomedical application examples, from single bacteria, tissues and cells in this two-part event.

Part 1: Introduction to multi-modal submicron O-PTIR/Raman/Fluorescence

• Challenges of current direct IR (FTIR & QCL) and Raman micro-spectroscopic techniques applied to the analysis of biological samples and how O-PTIR overcome all of these challenges
• Demonstration of sub-500nm IR spatial resolution
• Co-located sub-micron IR and fluorescence epi-widefield imaging
• Examples across a wide range of biological samples, such as cells, tissues and bacteria

Part 2: Asst Prof Davis – Live cell imaging by sub-micron IR: Tracking de novo lipogenesis in single cells with stable isotope probes

• Overview of de novo lipogenesis (DNL) and it’s role in many modern diseases such as cancer, obesity, diabetes and nonalcoholic fatty liver disease
• Challenges of lipid imaging using traditional techniques
• Use of sub-micron IR (O-PTIR) for the spatiotemporal tracking of DNL using stable isotope labelled glucose in live cells in regular water