Groot-Ammers | September 17th, 2026

The third educational tutorial on fluorescence spectroscopy is dedicated to time-resolved measurements, in particular lifetime analysis. The characterisation of photoluminescence phenomena over time is vital for a complete description of the excited-state dynamics.

DATE & TIME
📆 Tuesday, September 22nd, 2026
🕔 10-11 AM  or 5-6 PM (CET) 

This study is supported by relating photoluminescence intensity to the decay time of emitted photons, i.e. emission lifetime. Lifetime is independent of photobleaching and concentration, but is relevant when studying the same substances in different environments or probing various physical parameters, such as pH, viscosity, and even temperature. Time-resolved analysis is a very sensitive technique allowing the study of any photoluminescent species: materials and semiconductors, bio- and synthetic molecules, in solution or in solid-state.

Lifetime technics and analyses:

• Differences between Time- and Frequency-Domain techniques;
• Time-resolved Emission Spectra – TRES;
• Time-Correlated Single-Photon Counting for nanosecond lifetimes – TCSPC;
• Microsecond lifetime techniques: Multichannel Scaling – MCS, SSTD & boxcar;
• Applications for lifetime analysis: FRET, FLIM & Time-resolved Anisotropy.